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Chromatin Immunoprecititation
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大小:81 |
2010-08-09
张杰
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Tell me about your research for which you are using
the Mx3005P.
For the past 7 years our area of research has
focused on the mechanisms that control DNA
repair, using S. pombe as a model organism. We
use real time PCR on the Mx3005P to quantify
the amount of protein-bound DNA after immuno-
precipitation. We mainly look at the recruitment of
specific DNA repair proteins to known DNA target
sequences, namely telomeres, centromeres,
DNA nicks and replication fork pause sites. ChIP
comprises about 85% of the work we do on the
Mx3005P, whilst the remaining proportion of our
work is dedicated to gene expressiona.
the Mx3005P.
For the past 7 years our area of research has
focused on the mechanisms that control DNA
repair, using S. pombe as a model organism. We
use real time PCR on the Mx3005P to quantify
the amount of protein-bound DNA after immuno-
precipitation. We mainly look at the recruitment of
specific DNA repair proteins to known DNA target
sequences, namely telomeres, centromeres,
DNA nicks and replication fork pause sites. ChIP
comprises about 85% of the work we do on the
Mx3005P, whilst the remaining proportion of our
work is dedicated to gene expressiona.
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