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Fluorescent DNA Labeling by PC
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2009-01-13
张国厚
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Fluorescent DNA Labeling by PCR
Introduction
Amplification of DNA using the polymerase chain reaction
(PCR)* provides the opportunity to label the resulting product
with either modified nucleotides or oligonucleotide primers.
Incorporation of fluorescent labels offers the advantages of direct
detection, sensitivity, and multicolor capability. Both fluorescently
labeled deoxynucleotide triphosphates (dNTPs) and fluorescently
end-labeled oligonucleotide primers are available for use in PCR
product labeling.
Introduction
Amplification of DNA using the polymerase chain reaction
(PCR)* provides the opportunity to label the resulting product
with either modified nucleotides or oligonucleotide primers.
Incorporation of fluorescent labels offers the advantages of direct
detection, sensitivity, and multicolor capability. Both fluorescently
labeled deoxynucleotide triphosphates (dNTPs) and fluorescently
end-labeled oligonucleotide primers are available for use in PCR
product labeling.
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