Introduction The Agilent 5600LS AFM system features a fully addressable, 200 mm x 200 mm stage to support samples of various sizes. After loading a sample as large as an 8-inch wafer on the stage, any locations (including the wafer edge) can be reached directly by the AFM tip without remounting or rotating the sample. In addition, the 5600LS offers the ability to move the stage with sub- micrometer-scale precision over the system’s entire hundreds-of-millimeters translation range, thus enabling high- resolution tip positioning at selected sample locations. This application note provides an explanation, illustration, and how-to guide of high-resolution tip positioning and its role in facilitating in situ AFM imaging.