Abstract Jitter is increasingly analyzed by separating a signal’s timing noise into its random and deterministic components, yet there is no reference standard for measurement verification. We introduce a precisely calibrated jitter source capable of applying a wide variety of jitter signals - Gaussian random jitter, periodic jitter (including, but not limited to, sinusoidal jitter), inter-symbol interference, and duty cycle distortion – in different combinations at adjustable amplitudes. In most cases the calibration of the jitter sources is traceable to accepted standards with uncertainties close to 1%. We describe the system, the calibration techniques, and give examples of its utility.