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超细银- 金复合颗粒增强酶生物传感器的研究
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2009-07-14
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用琥珀酸二异辛酯磺酸钠/ 环己烷反胶束体系合成憎水纳米银- 金复合颗粒,并用此纳米银- 金颗粒与聚乙烯醇缩丁醛构成复合固酶膜基质,用溶胶- 凝胶法固定葡萄糖氧化酶,构建葡萄糖生物传感器. 实验表明,纳米憎水银- 金颗粒可以大幅度提高固定化酶的催化活性,响应电流从相应浓度的几十纳安增强到几万纳安. 探讨了纳米颗粒效应在固定化酶中所起的作用,为纳米颗粒在生物传感器领域中的应用提供了可供参考的实验和理论依据.
关键词 纳米憎水银- 金颗粒,固定化酶,葡萄糖氧化酶,生物传感器
Abstract Hydrophobic Ag2Au nanoparticles are produced in AOT [Aeroslo OT , sodium bis (22ethyhexyl ) sulfosuccinate ]2cyclohexane reverse micellar system. Glucose biosensors have been formed with glucose oxidase (GOD) immobilized in composite immobilization membrane matrix , which is composed of hydrophobic Ag2Au particles and polyvinyl butyral ( PVB) by a sol2gel method. The experiments show that nanoparticles can significantly enhance the catalytic activity of the immobilized enzyme. The current response is increased from tens of nanoamperometer (nA) to ten thousands of nanoamperometer to the same glucose concentration. The experiment and theory proved the function of nanoparticles in immobilization of enzyme. The catalysis of enzyme improved by nanoparticles was discussed. This research provides experimental and theoretical basis for preparation of useful glucose biosensor.
Keywords hydrophobic Ag2Au nanoparticles , immobilized enzyme , glucose oxidase , biosensor
关键词 纳米憎水银- 金颗粒,固定化酶,葡萄糖氧化酶,生物传感器
Abstract Hydrophobic Ag2Au nanoparticles are produced in AOT [Aeroslo OT , sodium bis (22ethyhexyl ) sulfosuccinate ]2cyclohexane reverse micellar system. Glucose biosensors have been formed with glucose oxidase (GOD) immobilized in composite immobilization membrane matrix , which is composed of hydrophobic Ag2Au particles and polyvinyl butyral ( PVB) by a sol2gel method. The experiments show that nanoparticles can significantly enhance the catalytic activity of the immobilized enzyme. The current response is increased from tens of nanoamperometer (nA) to ten thousands of nanoamperometer to the same glucose concentration. The experiment and theory proved the function of nanoparticles in immobilization of enzyme. The catalysis of enzyme improved by nanoparticles was discussed. This research provides experimental and theoretical basis for preparation of useful glucose biosensor.
Keywords hydrophobic Ag2Au nanoparticles , immobilized enzyme , glucose oxidase , biosensor
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