Ever since its invention, atomic force microscopy (AFM) has revolutionized the field of interfacial surface science by enabling direct, high-resolution visualization of surface morphology in various solutions and gas environments. With the advent of new technologies that simplify the preparation and handling of samples, the use of AFM is becoming increasingly widespread in biological research. The potential of Agilent’s MAC Mode AFM for accelerating drug development is also tremendous. Highlighted below are a few examples of the many MAC Mode applications performed under ambient conditions, in solvents, or under other controlled conditions (e.g., 37 °C) using Agilent atomic force microscopes.