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Signal Transduction
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2010-08-08
djelje
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Linking Gene Discovery to Function
As the research focus shifts from gene discovery to functional
genomics, scientists need to link genes and proteins to their
biological processes. The PathDetectÆ
Pathway Profiling System
measures transcription activation quantitatively and with greater
sensitivity than cumbersome blotting or gel-shift assays. Previously,
tedious blotting procedures were required to monitor transcription
of the cellular genes that indicate pathway activation. Alternatively,
researchers used time-consuming, non-quantitative gel-shift assays
(EMSA) to measure transcription factor-binding activity. With the
PathDetect products, reporter enzymes replace cellular genes, and
simple enzyme assays provide readout of transcription activation.
As the research focus shifts from gene discovery to functional
genomics, scientists need to link genes and proteins to their
biological processes. The PathDetectÆ
Pathway Profiling System
measures transcription activation quantitatively and with greater
sensitivity than cumbersome blotting or gel-shift assays. Previously,
tedious blotting procedures were required to monitor transcription
of the cellular genes that indicate pathway activation. Alternatively,
researchers used time-consuming, non-quantitative gel-shift assays
(EMSA) to measure transcription factor-binding activity. With the
PathDetect products, reporter enzymes replace cellular genes, and
simple enzyme assays provide readout of transcription activation.
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